Recombinant Filaggrin antibody (AA 198-288)

Catalog No. ABIN7126403

This Mouse Monoclonal antibody specifically detects Filaggrin in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant Filaggrin antibody (AA 198-288) (ABIN7126403)

Target: Filaggrin (FLG)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Filaggrin Antibody

Binding Specificity: AA 198-288

Specificity: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-251-MGor A-431 cells. Human skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: FLG

Background: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin,Filaggrin (Keratinocyte Differentiation Marker)
Cellular localisation: Cytoplasm

Molecular Weight: 26-45kDa (Processed), 350kDa (Profilaggrin)

Gene ID: 2312, 654510

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance