CSTF2T antibody

Catalog No. ABIN7126415

Product Details anti-CSTF2T Antibody

Target: CSTF2T (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa, tau Variant (CSTF2T))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSTF2T antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CSTF2T protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A375, Jurkat or HeLa cells. Human colon adenocarcinoma or lung adenocarcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CSTF2T

Target: CSTF2T (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa, tau Variant (CSTF2T))

Alternative Name: CSTF2T (CSTF2T Products)

Background: CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 3' pre RNA, subunit 2 64kDa tau variant; Cleavage stimulation factor 64 kDa subunit tau; Cleavage stimulation factor subunit 2 tau variant; CSTF 64 kDa subunit tau,CSTF2T (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 64kDa

Gene ID: 23283, 591358

UniProt: Q9H0L4