Recombinant FSHB antibody

Catalog No. ABIN7126442

This Rabbit Monoclonal antibody specifically detects FSHB in WB, FACS, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant FSHB antibody (ABIN7126442)

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FSHB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-FSHB Antibody

Specificity: This MAb reacts with a protein of 22 kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant hFSH beta sub-unit

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Anterior Pituitary

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FSHB

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Alternative Name: FSHB

Background: Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin subunit beta, FSH-B, FSH-beta, FSHB, FSHbeta,FSH-beta (Follicle Stimulating Hormone-beta)
Cellular localisation: Cytoplasmic, secreted

Molecular Weight: 22kDa

Gene ID: 2488, 36975

UniProt: P01225

Pathways: Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process