GCLM antibody

Catalog No. ABIN7126478

This Mouse Monoclonal antibody specifically detects GCLM in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for GCLM antibody (ABIN7126478)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-GCLM Antibody

Specificity: GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length GCLM protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background: GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody,Glutamate-Cysteine Ligase Regulatory Subunit
Cellular localisation: Cytoplasm.

Molecular Weight: 31kDa

Gene ID: 2730, 315562

UniProt: P48507