GTF2A1 antibody

Catalog No. ABIN7126495

This anti-GTF2A1 antibody is a Mouse Monoclonal antibody detecting GTF2A1 in ELISA, IP, IF, Coat and FACS. Suitable for Human.

Quick Overview for GTF2A1 antibody (ABIN7126495)

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GTF2A1 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)

Product Details anti-GTF2A1 Antibody

Specificity: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-i±, TFIIA-i² and TFIIA-i³, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.

Cross-Reactivity (Details): Human. Predicted to react in Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GTF2A1 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF-7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GTF2A1

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Alternative Name: GTF2A1

Background: General transcription factor IIA 1 19/37 kDa, General transcription factor IIA subunit 1, Glucose regulated protein 58kD pseudogene, TFIIA alpha p55, isoform 1, TFIIA p19 subunit, TFIIA p35 subunit, TFIIAL, Transcription initiation factor IIA beta chain, Transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit,GTF2A1/ TFIIA (Transcription Factor)
Cellular localisation: Nucleus

Molecular Weight: 41.51kDa

Gene ID: 2957, 592334

UniProt: P52655