GTF2B antibody
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- Target See all GTF2B Antibodies
- GTF2B (General Transcription Factor IIB (GTF2B))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GTF2B antibody is un-conjugated
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Application
- ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)
- Specificity
- In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human GTF2Bprotein
- Isotype
- IgG2b
- Top Product
- Discover our top product GTF2B Primary Antibody
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- Application Notes
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Known_Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or MCF-7 cells.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- GTF2B (General Transcription Factor IIB (GTF2B))
- Alternative Name
- GTF2B (GTF2B Products)
- Synonyms
- CG5193 antibody, Dmel\\CG5193 antibody, TFIIB antibody, Tfiib antibody, TfllB antibody, dTFIIB antibody, dTfIIB antibody, dmTFIIB antibody, gtf2b antibody, tf2b antibody, tfiib antibody, XLTFIIB antibody, TF2B antibody, wu:fc44c11 antibody, zgc:66258 antibody, Transcription factor IIB antibody, transcription initiation factor iib antibody, transcription initiation factor IIB antibody, transcription initiation factor IIB 2 antibody, Transcription initiation factor IIB antibody, general transcription factor 2B S homeolog antibody, general transcription factor 2B L homeolog antibody, general transcription factor IIB antibody, TfIIB antibody, CNF01950 antibody, tfb antibody, CC1G_14689 antibody, HacjB3_18813 antibody, HALXA_RS07110 antibody, HALXA_RS16310 antibody, tf2b antibody, LOC100283348 antibody, gtf2b.S antibody, gtf2b.L antibody, GTF2B antibody, gtf2b antibody, Gtf2b antibody
- Background
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General transcription factor IIB, General transcription factor TFIIB, gtf2b, RNA polymerase II transcription factor IIB, S300 II, S300-II, TF IIB, TF2B, TFIIB, Transcription initiation factor IIB,GTF2B/ TFIIB (Transcription Factor 2B)
Cellular localisation: Nucleus. Nucleoplasm, nuclear bodies. - Molecular Weight
- 34.8kDa
- Gene ID
- 2959, 481852
- UniProt
- Q00403
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