GTF2B antibody

Catalog No. ABIN7126496

This Mouse Monoclonal antibody specifically detects GTF2B in ELISA, IP, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for GTF2B antibody (ABIN7126496)

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GTF2B antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-GTF2B Antibody

Specificity: In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GTF2Bprotein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF-7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GTF2B

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: GTF2B

Background: General transcription factor IIB, General transcription factor TFIIB, gtf2b, RNA polymerase II transcription factor IIB, S300 II, S300-II, TF IIB, TF2B, TFIIB, Transcription initiation factor IIB,GTF2B/ TFIIB (Transcription Factor 2B)
Cellular localisation: Nucleus. Nucleoplasm, nuclear bodies.

Molecular Weight: 34.8kDa

Gene ID: 2959, 481852

UniProt: Q00403