HDAC1 antibody

Catalog No. ABIN7126509

The Mouse Monoclonal anti-HDAC1 antibody has been validated for ELISA, IF, IP, FACS and Coat. It is suitable to detect HDAC1 in samples from Human.

Quick Overview for HDAC1 antibody (ABIN7126509)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-HDAC1 Antibody

Specificity: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human HDAC1 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: DKFZp686H12203, GON 10, HD1, Reduced potassium dependency yeast homolog like 1 (RPD3L1), RPD3,Histone Deacetylase 1 (HDAC1)
Cellular localisation: Nucleus. Nucleoplasm.

Molecular Weight: 60kDa

Gene ID: 3065, 88556

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis