HOXB2 antibody (AA 114-220)

Catalog No. ABIN7126513

This anti-HOXB2 antibody is a Mouse Monoclonal antibody detecting HOXB2 in WB, ELISA, IP, IF, Coat, FACS and IHC (f). Suitable for Human.

Quick Overview for HOXB2 antibody (AA 114-220) (ABIN7126513)

Target: HOXB2 (Homeobox B2 (HOXB2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HOXB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-HOXB2 Antibody

Binding Specificity: AA 114-220

Specificity: Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxB1 is critical to hindbrain development and has phenotypic features frequently observed in autism. Analysis of expression and targeted disruption of HoxB1 demonstrates that it is also essential for patterning progenitor cells along the entire DV axis of rhombomere 4 (r4). HoxB1 maintains this function by acting very early during hindbrain neurogenesis to specify effectors of the Sonic hedgehog and Mash1 signaling pathways. HoxB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis, hindbrain development and normal human adult lung development. HoxB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation. In addition, HoxB2 plays an important role in the patterning of hindbrain and pharyngeal arches in the zebrafish.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa114-220) of human HOXB2

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HOXB2

Target: HOXB2 (Homeobox B2 (HOXB2))

Alternative Name: HOXB2

Background: Homeo box 2H, homeo box B2, homeobox B2, Homeobox protein Hox-2.8, Homeobox protein Hox-2H, Homeobox protein Hox-B2, Hox 2 8, Hox 2H, HOX2, HOX2H, hoxb2, HXB2_HUMAN, K8, K8 home protein,HOXB2 (Transcription Factor)
Cellular localisation: Nucleus. Nucleoplasm.

Molecular Weight: 37.9kDa

Gene ID: 3212, 514289

UniProt: P14652