Apolipoprotein D antibody (AA 21-119)

Catalog No. ABIN7126541

This Mouse Monoclonal antibody specifically detects Apolipoprotein D in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Apolipoprotein D antibody (AA 21-119) (ABIN7126541)

Target: Apolipoprotein D (APOD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Apolipoprotein D antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Apolipoprotein D Antibody

Binding Specificity: AA 21-119

Specificity: Lipids, such as phospholipids, triacylglycerols and cholesterol, are weakly soluble in aqueous solution and therefore are transported by circulation as components of lipoproteins. Lipoproteins are globular particles that consist of a non-polar core of triacylglycerols and cholesteryl esters surrounded by phospholipid, cholesterol and an amphiphilic coating of protein, known as apolipoproteins (apo). These complexes allow the dissolution and shuttling of their non-polar lipid components. At least nine different apolipoproteins are distributed in significant amounts in different human lipoproteins. Apolipoprotein D (apoD) is a member of the lipocalin superfamily of transporter proteins that bind small hydrophobic molecules, including arachidonic acid (AA). The ability of apoD to bind AA implicates it in pathways associated with membrane phospholipid signal transduction and metabolism. apoD expression has been shown to correlate both with cell cycle arrest and with prognosis in several types of malignancy, including central nervous system astrocytomas and medulloblastomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human recombinant APOD protein fragment (around aa 21-119) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human colon and breast cancer tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Apolipoprotein D

Target: Apolipoprotein D (APOD)

Alternative Name: APOD

Background: APO-D protein,Apolipoprotein D / APOD
Cellular localisation: Secreted.

Molecular Weight: 30kDa

Gene ID: 347, 522555

UniProt: P05090

Pathways: Platelet-derived growth Factor Receptor Signaling