Inhibin alpha antibody (AA 233-362)

Catalog No. ABIN7126589

This Mouse Monoclonal antibody specifically detects Inhibin alpha in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Inhibin alpha antibody (AA 233-362) (ABIN7126589)

Target: Inhibin alpha (INHA) (Inhibin, alpha (INHA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Inhibin alpha antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Inhibin alpha Antibody

Binding Specificity: AA 233-362

Specificity: It recognizes a 47 kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same i± subunit, covalently linked to 1 of 2 distinct subunits, i²-i± or i²-i². Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor i² (TFGi²) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human Inhibin alpha protein (around aa 233-362) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or testis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Inhibin alpha

Target: Inhibin alpha (INHA) (Inhibin, alpha (INHA))

Alternative Name: INHA

Background: A inhibin subunit, IHA, inhA, Inhibin alpha chain, Inhibin alpha subunit,Inhibin, alpha (INHA) (Gonadal Cell Marker)
Cellular localisation: Nuclear and Cytoplasmic (secreted)

Molecular Weight: 47kDa

Gene ID: 3623, 407506

UniProt: P05111

Pathways: Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion