Keratin 3 antibody

Catalog No. ABIN7126611

The Mouse Monoclonal anti-Keratin 3 antibody has been validated for WB and IHC (f). It is suitable to detect Keratin 3 in samples from Rabbit.

Quick Overview for Keratin 3 antibody (ABIN7126611)

Target: Keratin 3 (KRT3)

Reactivity: Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Keratin 3 Antibody

Specificity: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Cross-Reactivity (Details): Human, rabbit and bovine.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Human cornea.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Keratin 3

Target: Keratin 3 (KRT3)

Alternative Name: KRT3

Background: 65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3,Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 65kDa

Gene ID: 38550, 680652

UniProt: P12035