Recombinant KRT14 antibody (AA 350-472)

Catalog No. ABIN7126632

The Rabbit Monoclonal anti-KRT14 antibody has been validated for IHC (f). It is suitable to detect KRT14 in samples from Human.

Quick Overview for Recombinant KRT14 antibody (AA 350-472) (ABIN7126632)

Target: KRT14 (Keratin 14 (KRT14))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-KRT14 Antibody

Binding Specificity: AA 350-472

Specificity: Cytokeratin 14 is a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia.Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated in the differentiating suprabasal cell layers. Otherwise, in the widely well stratified follicular outer root sheath, cytokeratin 14 is uniformly expressed throughout all layers. The expression spectrum of cytokeratin 14 in tumors corresponds well to the patterns in normal epithelia. Thus, most squamous cell carcinomas as well as malignant mesotheliomas strongly express this keratin whereas little, focal, or no expression is found in adenocarcinomas.Cytokeratin 14 may be a useful marker in the differential diagnosis of squamous cell carcinoma from other epithelial tumors. Recent studies also indicate that CK14 expression in breast cancer corresponded with poor clinical outcome and that CK14 may have diagnostic value in the sub-classification of NSCLC.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 orHeLacells. Human prostate, skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: KRT14

Background: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ,Cytokeratin 14 (KRT14) (Squamous Cell Marker)
Cellular localisation: Cytoplasm.

Molecular Weight: 50kDa

Gene ID: 3861, 6545380

UniProt: P02533