MITF antibody

Catalog No. ABIN7126695

The Mouse Monoclonal anti-MITF antibody has been validated for WB, IF, FACS and IP. It is suitable to detect MITF in samples from Human.

Quick Overview for MITF antibody (ABIN7126695)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MITF Antibody

Specificity: MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

Cross-Reactivity (Details): Human. Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MITF protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Immunofluorescence (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells.Human melanoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A,Microphthalmia Transcription Factor (MITF)
Cellular localisation: Nucleus.

Molecular Weight: 52-56kDa (doublet)

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding