LMYC antibody

Catalog No. ABIN7126732

The Mouse Monoclonal anti-LMYC antibody (ABIN7126732) specifically detects LMYC in WB, IP, ELISA, IF, Coat and FACS. The antibody is reactive with Human samples.

Quick Overview for LMYC antibody (ABIN7126732)

Target: LMYC (MYCL) (Protein L-Myc (MYCL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LMYC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)

Product Details anti-LMYC Antibody

Specificity: Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas, and the L-Myc gene in human small cell lung carcinomas. c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MYCL protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human small cell lung carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for LMYC

Target: LMYC (MYCL) (Protein L-Myc (MYCL))

Alternative Name: MYCL

Background: BHLHe38, Class E basic helix-loop-helix protein 38, l myc 1 proto-oncogene, Myc related gene from lung cancer, oncogene lmyc, Protein L-Myc-1, v myc avian myelocytomatosis viral oncogene homolog 1 lung carcinoma derived,L-Myc / MYCL1 (Transcription Factor)
Cellular localisation: Nucleus. Nucleoplasm. Mitotic chromosome.

Molecular Weight: 40.33kDa

Gene ID: 4610, 437922

UniProt: P12524

Pathways: Warburg Effect