PBX1 antibody

Catalog No. ABIN7126761

Product Details anti-PBX1 Antibody

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PBX1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP), Coating (Coat)

Specificity: Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1,19)(q23,p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92 % and 94 % respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PBX1 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Expressed in all tissues except in cells of the B and T lineage.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PBX1

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Alternative Name: PBX1 (PBX1 Products)

Background: DKFZp686B09108, Homeobox protein PBX 1, Homeobox protein PBX1, Homeobox protein PRL, Pbx1, Pre B cell leukemia homeobox 1, Pre B cell leukemia transcription factor 1, Pre-B-cell leukemia transcription factor 1, PRL,PBX1 / PRL (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 86.1kDa

Gene ID: 5087, 557097

UniProt: P40424