PDGFB antibody (AA 27-158)

Catalog No. ABIN7126776

The Mouse Monoclonal anti-PDGFB antibody has been validated for IHC (f). It is suitable to detect PDGFB in samples from Human.

Quick Overview for PDGFB antibody (AA 27-158) (ABIN7126776)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFB antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-PDGFB Antibody

Binding Specificity: AA 27-158

Specificity: PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, i±and i², which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to i± receptors with high affinity, whereas the B subunit can bind to both i± and i² receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human PDGFB protein (around aa 27-158) (exact sequence is proprietary)

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human colon, kidney or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGFB

Background: Becaplermin, c sis, Oncogene SIS, PDGF Beta chain, PDGF2, Platelet derived growth factor 2, Platelet derived growth factor B chain, Platelet derived growth factor beta, Platelet derived growth factor beta polypeptide (oncogene SIS), Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog),PDGF beta (PDGFB)
Cellular localisation: Cytoplasm.

Molecular Weight: 14kDa (monomeric B chain), 31-35kDa (dimer)

Gene ID: 5155, 1976

UniProt: P01127

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling