Recombinant CD31 antibody (AA 28-330)

Catalog No. ABIN7126783

This anti-CD31 antibody is a Rabbit Monoclonal antibody detecting CD31 in IHC (f). Suitable for Human.

Quick Overview for Recombinant CD31 antibody (AA 28-330) (ABIN7126783)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD31 Antibody

Binding Specificity: AA 28-330

Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Cross-Reactivity (Details): Human. Weakly reacts with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 or Jurkat cells. Human tonsil or angiosarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background: EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: ~100kDa (endothelium) and ~130kDa (platelets)

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization