POLE3 antibody

Catalog No. ABIN7126801

This anti-POLE3 antibody is a Mouse Monoclonal antibody detecting POLE3 in IF, FACS and IHC (f). Suitable for Human.

Quick Overview for POLE3 antibody (ABIN7126801)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-POLE3 Antibody

Specificity: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Cross-Reactivity (Details): Human. Predicted to react in Mouse, Rat and Xenopus.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human POLE3 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: POLE3

Background: Arsenic transactivated protein, ASTP, CHRAC17, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, Histone fold protein CHRAC17, HuCHRAC17, Polymerase (DNA directed) epsilon 3 (POLE3), YBL1,POLE3 / CHRAC17
Cellular localisation: Nucleus. Cytoplasm.

Molecular Weight: 16.86kDa

Gene ID: 54107, 108112

UniProt: Q9NRF9

Pathways: DNA Damage Repair