Recombinant KRT20 antibody (AA 196-323)

Catalog No. ABIN7126808

This Mouse Monoclonal antibody specifically detects KRT20 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT20 antibody (AA 196-323) (ABIN7126808)

Target: KRT20 (Keratin 20 (KRT20))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-KRT20 Antibody

Binding Specificity: AA 196-323

Specificity: This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HT29 cells. Human normal colon or colon adenocarcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20

Background: CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Cellular localisation: Cytoplasm.

Molecular Weight: 46kDa

Gene ID: 54474, 84905

UniProt: P35900