FBLIM1 antibody

Catalog No. ABIN7126817

This Mouse Monoclonal antibody specifically detects FBLIM1 in WB, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for FBLIM1 antibody (ABIN7126817)

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBLIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-FBLIM1 Antibody

Specificity: Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human FBLIM1 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 or MCF7 cells. Human heart or kidney tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FBLIM1

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Alternative Name: FBLIM1

Background: CSX associated LIM (Cal), Filamin binding LIM protein 1 (FBLIM1), Filamin-binding LIM protein 1 (FBLP1), Gt10, MIG2-interacting protein, Migfilin, Mitogen inducible 2 interacting protein, Mitogen-inducible 2-interacting protein,Migfilin-1 / FBLIM1
Cellular localisation: Cytoplasm.

Molecular Weight: 41kDa

Gene ID: 54751, 530101

UniProt: Q8WUP2