PRMT6 antibody

Catalog No. ABIN7126821

The Mouse Monoclonal anti-PRMT6 antibody has been validated for IF, IP and FACS. It is suitable to detect PRMT6 in samples from Human.

Quick Overview for PRMT6 antibody (ABIN7126821)

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRMT6 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-PRMT6 Antibody

Specificity: A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMTs contain an S-adenosylmethione motif which functions to add one or two methyl groups to guanidino nitrogens of arginine (R) side chains. PRMT6, also known as HRMT1L6, is a nuclear protein belonging to the PRMT family and is predominantly expressed in testis and kidney. It is known to methylate Histones H3, H4 and H2A. PRMT6 is the major dimethyltransferase for Histone H3 and specifically methylates Histone H3 at R2. Methylation at Histone H3 R2 acts to inhibit Histone H3 K4 trimethylation and ultimately leads to the transcriptional repression of genes that are activated by Histone H3 K4 trimethylation. In addition, PRMT6 methylates HIV TAT, possibly functioning as a form of cellular innate immunity to restrict levels of HIV replication.

Cross-Reactivity (Details): Human. Predicted to react with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PRMT6 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PRMT6

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Alternative Name: PRMT6

Background: Chromobox protein homolog 7, Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6, Histone-arginine N-methyltransferase PRMT6, HMT1 hnRNP methyltransferase like 6., HRMT1L6,PRMT6
Cellular localisation: Nucleus.

Molecular Weight: 42kDa

Gene ID: 55170, 26006

UniProt: Q96LA8