FERMT1 antibody (AA 216-677)

Catalog No. ABIN7126830

The Mouse Monoclonal anti-FERMT1 antibody is suitable to detect FERMT1 in samples from Human. It has been validated for WB, IP and IHC (f).

Quick Overview for FERMT1 antibody (AA 216-677) (ABIN7126830)

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FERMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-FERMT1 Antibody

Binding Specificity: AA 216-677

Specificity: Kindlin-1 (also known as KIND1, KINDLERIN, UNC112-RELATED PROTEIN 1 (URP1) is an important component of cell-extracellular matrix adhesion. Kindlin-1 is involved in cell adhesion, possibly via its interaction with integrins and may mediate TGF-beta 1 signaling during tumor progression. Kindlin-1 has been shown to be significantly upregulated in lung and colon carcinomas. Mutations in the kindlin-1 gene, FERMT1, causes Kindler syndrome. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. We have developed monoclonal anti-kidlin-1 antibodies. This monoclonal antibody recognizes kindlin-1 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant protein containing Kindlin-1 (residues 216-677)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 293T whole cell lysate. Human tonsil or liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FERMT1

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Alternative Name: FERMT1

Background: Fermitin family homolog 1 (Fermt1), Kindlin-1 (KIND1), Kindlerin, Unc-112, UNC112A, URP1, DTGCU2,Kindlin-1 / KIND1
Cellular localisation: Cytoplasm.

Molecular Weight: 77kDa

Gene ID: 55612, 472054

UniProt: Q9BQL6