PKC alpha antibody (C-Term)
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- Target See all PKC alpha (PKCa) Antibodies
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PKC alpha antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Protein Kinase C alpha (PKCi±) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the i± isoform of PKCi± and binds to a sequence at the C terminus of PKCi± to detect its expression in vitro.
- Cross-Reactivity (Details)
- Human, Rat and Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCα.
- Isotype
- IgG2a
- Top Product
- Discover our top product PKCa Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Normal human kidney or tonsil tissue. HEK293 whole cell lysates.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- Alternative Name
- PRKCA (PKCa Products)
- Synonyms
- pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody
- Background
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PKC alpha; PKC-A; PKC-alpha; PKCA; PRKCA; Protein kinase C alpha; Protein kinase C alpha type,Protein Kinase C α / PRKCA
Cellular localisation: Cytoplasm. Cell Membrane. Nucleus. - Molecular Weight
- 77kDa
- Gene ID
- 5578, 531704, 708867
- UniProt
- P17252
- Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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