S100A1 antibody

Catalog No. ABIN7126910

This Rabbit Monoclonal antibody specifically detects S100A1 in IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for S100A1 antibody (ABIN7126910)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This S100A1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-S100A1 Antibody

Specificity: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human S-100 protein conjugated to methylated BSA

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: T98G, A431 or HT-29 cells. Human brain or melanoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1

Background: Bpb, NEF, S100-A1, S100 Alpha Chain, S100 Beta Chain, S100 Calcium Binding Protein A1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein Beta Neural,S100A1 (Melanoma Marker)
Cellular localisation: Cytoplasm

Molecular Weight: 10-12kDa

Gene ID: 6271, 515715

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins