S100B antibody (AA 3-92)
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- Target See all S100B Antibodies
- S100B (S100 Calcium Binding Protein B (S100B))
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Binding Specificity
- AA 3-92
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This S100B antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa3-92) of human S100B protein (exact sequence is proprietary)
- Isotype
- IgG2b
- Top Product
- Discover our top product S100B Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human brain or melanoma tissue.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- S100B (S100 Calcium Binding Protein B (S100B))
- Alternative Name
- S100B (S100B Products)
- Synonyms
- NEF antibody, S100 antibody, S100-B antibody, S100beta antibody, S100P antibody, AI850290 antibody, Bpb antibody, si:dkey-110p14.2 antibody, wu:fq18b10 antibody, zgc:92776 antibody, S100 calcium binding protein B antibody, S100 protein, beta polypeptide, neural antibody, S100 calcium binding protein, beta (neural) antibody, S100B antibody, S100b antibody, s100b antibody
- Background
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NEF, Protein S100-B, S-100 protein beta chain, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100beta,S100B (Astrocyte and Melanoma Marker)
Cellular localisation: Cytoplasmic and Nuclear - Molecular Weight
- 10-12kDa
- Gene ID
- 6285, 422181
- UniProt
- P04271
- Pathways
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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