Recombinant SOX11 antibody (AA 300-400)

Catalog No. ABIN7126964

This anti-SOX11 antibody is a Rabbit Monoclonal antibody detecting SOX11 in IHC (f). Suitable for Human.

Quick Overview for Recombinant SOX11 antibody (AA 300-400) (ABIN7126964)

Target: SOX11 (SRY (Sex Determining Region Y)-Box 11 (SOX11))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SOX11 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SOX11 Antibody

Binding Specificity: AA 300-400

Specificity: Recognizes a protein of ~47 kDa, identified as SOX11. This MAb is highly specific and does not cross-react with other members of the SOX-family. Mantle cell lymphoma (MCL) accounts for 5 % to 10 % of mature B-cell neoplasms and is an aggressive disease genetically characterized by overexpression of Cyclin D1. Cyclin D1 overexpression is the hallmark of MCL. However, approximately 5 %-10 % of MCLs lack Cyclin D1 expression and may be misdiagnosed. Almost all Cyclin D1-positive as well as Cyclin D1-negative MCL, show overexpression of SOX-11. The detection of this transcription factor is a useful biomarker for identifying true cyclin D1-negative MCL.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa300-400) of human SOX11 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 or Y79 cells. Human cervix or mantle cell lymphoma (MCL).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX11

Target: SOX11 (SRY (Sex Determining Region Y)-Box 11 (SOX11))

Alternative Name: SOX11

Background: SOX11, SRY (sex determining region Y) box 11, SRY related HMG box gene 11, Transcription factor SOX-11,SOX11 (Mantle Cell Lymphoma Marker)
Cellular localisation: Nuclear (Cervix), Cytoplasmic (Melanoma)

Molecular Weight: 47kDa

Gene ID: 6664, 432638

UniProt: P35716

Pathways: Tube Formation