MED22 antibody

Catalog No. ABIN7126989

This Mouse Monoclonal antibody specifically detects MED22 in ELISA, IF, Coat, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for MED22 antibody (ABIN7126989)

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED22 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MED22 Antibody

Specificity: Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MED22 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunofluorescence (0.5-2 μg/mL), Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (0.5-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MED22

Target: MED22 (Mediator Complex Subunit 22 (MED22))

Alternative Name: MED22

Background: Med24, SURF5, mediator complex subunit 22, Mediator of RNA polymerase II transcription subunit 22,MED22 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 22kDa

Gene ID: 6837, 78354

UniProt: Q15528

Pathways: Regulation of Lipid Metabolism by PPARalpha