TARBP2 antibody

Catalog No. ABIN7126994

This anti-TARBP2 antibody is a Mouse Monoclonal antibody detecting TARBP2 in WB, IF and FACS. Suitable for Human.

Quick Overview for TARBP2 antibody (ABIN7126994)

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TARBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TARBP2 Antibody

Specificity: TRBP2, also known as TARBP2 (trans-activation-responsive (HIV-1) RNA binding protein 2), TRBP1 or TRBP, is a nuclear protein that contains three DRBM (double-stranded RNA-binding) domains. TRBP binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. The third DRBM motif in the C-terminus of human TRBP2 can interact with and inhibit PKR activity, thereby increasing HIV-1 long terminal repeat (LTR) expression. In addition, TRBP2 functions as a component of a Dicer-containing complex and associates with the catalytic subunit of the RNA-induced silencing complex (RISC), namely eIF2C2. TRBP2 is essential for Dicer stability and the proper assembly of RISC. This suggests that TRBP2, in association with Dicer, plays an important role in the processing of miRNAs (microRNAs).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human TARBP2 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TARBP2

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Alternative Name: TARBP2

Background: LOQS, Prbp, RISC loading complex subunit TARBP2, TAR (HIV 1) RNA binding protein 2, TAR RNA Binding Protein 2, TARBP2 RISC loading complex RNA binding subunit, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1,TRBP2 / TARBP2
Cellular localisation: Cytoplasm. Cytoplasm > perinuclear region. Nucleus.

Molecular Weight: 45kDa

Gene ID: 6895, 326

UniProt: Q15633

Pathways: Regulatory RNA Pathways, Ribonucleoprotein Complex Subunit Organization