UBE2B antibody

Catalog No. ABIN7127038

The Mouse Monoclonal anti-UBE2B antibody is suitable to detect UBE2B in samples from Human. It has been validated for IF and FACS.

Quick Overview for UBE2B antibody (ABIN7127038)

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2B antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-UBE2B Antibody

Specificity: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Cross-Reactivity (Details): Human. Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human UBE2B protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, K562 or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for UBE2B

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Alternative Name: UBE2B

Background: E2 17 kDa, hHR6B, HR6B, RAD6 homolog B (RAD6B), UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2B, Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa,UBE2B
Cellular localisation: Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.

Molecular Weight: 17kDa

Gene ID: 7320

UniProt: P63146