Recombinant WT1 antibody (AA 1-100)

Catalog No. ABIN7127056

The Mouse Monoclonal anti-WT1 antibody has been validated for IHC (f). It is suitable to detect WT1 in samples from Human.

Quick Overview for Recombinant WT1 antibody (AA 1-100) (ABIN7127056)

Target: WT1 (Wilms Tumor 1 (WT1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-WT1 Antibody

Binding Specificity: AA 1-100

Specificity: The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62 kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus. WT1 influences cell proliferation by suppressing bcl-2 and regulating cadherin and p53. In normal epithelia, nuclear WT1 expression is largely restricted to ovary (surface epithelium and inclusion cysts) and fallopian tube, while WT1 is not found in endometrial or cervical epithelium. As regards nonepithelial cells, nuclear WT1 is found in mesothelium and some sub mesothelial stromal cells, stromal cells of the female genital tract, testicular non-germinal cells, and kidney (podocytes). In tumor tissues, WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas.WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from nonserous carcinomas. As for malignant mesothelioma, calretinin and WT1 are superior to cytokeratin 5/6, N-cadherin and thrombomodulin. WT1 is also applicable for the differential diagnostic of small cell childhood tumors.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells. Human Wilm s tumor, mesothelioma or fetal kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1,Wilm s Tumor 1 (WT1) (Wilm s Tumor & Mesothelial Marker)
Cellular localisation: Nucleus and cytoplasm. Shuttles between nucleus and cytoplasm.

Molecular Weight: 49kDa

Gene ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation