CA9 antibody (AA 314-410)

Catalog No. ABIN7127066

This anti-CA9 antibody is a Mouse Monoclonal antibody detecting CA9 in IHC, FACS and IHC (f). Suitable for Human.

Quick Overview for CA9 antibody (AA 314-410) (ABIN7127066)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CA9 Antibody

Binding Specificity: AA 314-410

Specificity: Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CA9 protein fragment (around aa 314-410) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U87 cells. Normal kidney or renal cell carcinoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9

Background: CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250,Renal Cell Carcinoma (Carbonic Anhydrase IX)
Cellular localisation: Cell Surface. Cytoplasmic.

Molecular Weight: 55kDa

Gene ID: 768, 63287

UniProt: Q16790