HDAC3 antibody

Catalog No. ABIN7127118

This Mouse Monoclonal antibody specifically detects HDAC3 in WB, ELISA, IF, IP, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for HDAC3 antibody (ABIN7127118)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-HDAC3 Antibody

Specificity: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human HDAC3 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: HD3, HDAC 3, HDAC3, HDAC3_HUMAN, Histone deacetylase 3, RPD3 2, RPD3, RPD3-2, SMAP45,Histone Deacetylase 1 (HDAC3)
Cellular localisation: Nucleus. Nucleoplasm. Golgi apparatus.

Molecular Weight: 49kDa

Gene ID: 8841, 519632

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development