CD5L antibody (AA 269-366)

Catalog No. ABIN7127146

The Mouse Monoclonal anti-CD5L antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect CD5L in samples from Human.

Quick Overview for CD5L antibody (AA 269-366) (ABIN7127146)

Target: CD5L (CD5 Molecule-Like (CD5L))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5L antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD5L Antibody

Binding Specificity: AA 269-366

Specificity: CD5L (CD5 Molecule-like), also known as API6, PRO229, Sp or SP-ALPHA, is a 347 amino acid secreted protein that belongs to the scavenger receptor cysteine-rich (SRCR) family of leukocyte regulating proteins. Expressed in bone marrow, spleen, thymus, lymph node and fetal liver, CD5L is thought to be involved in regulating the immune system via binding to peripheral monocytes and mediating their activation and overall survival. CD5L has three cysteine-rich domains and, in addition to its role in the immune system, may function to inhibit apoptosis and promote macrophage survival.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Ramos, or MOLT-4 cells. Tonsil or Lymph Node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD5L

Target: CD5L (CD5 Molecule-Like (CD5L))

Alternative Name: CD5L

Background: AAC-11, AIM, API6, APOPTOSIS INHIBITOR 6, APOPTOSIS INHIBITOR OF MACROPHAGES, CD5 antigen like (scavenger receptor cysteine rich family), CD5 antigen-like, CT-2, Highly similar to ANTIGEN WC1.1 [Bos taurus], IgM-associated peptide, Pdp, PRO229, SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY, SP-alpha, Spalpha,CD5L / CD5LG / CD5 Ligand
Cellular localisation: Secreted.

Molecular Weight: 38kDa

Gene ID: 922, 134035

UniProt: O43866