MED21 antibody

Catalog No. ABIN7127181

This Mouse Monoclonal antibody specifically detects MED21 in WB, ELISA, IF, Coat, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for MED21 antibody (ABIN7127181)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MED21 Antibody

Specificity: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MED21 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: MED21

Background: Forkhead box protein Q1, MED21, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH1, HNF3/forkhead like protein 1, MGC77469, Q1, Winged helix / forkhead transcription factor, zgc:77469,MED21 / SRB7
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molecular Weight: 15.5kDa

Gene ID: 9412, 286145

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha