MED21 antibody (N-Term)

Catalog No. ABIN2776418

The Rabbit Polyclonal anti-MED21 antibody is suitable to detect MED21 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB and ChIP.

Quick Overview for MED21 antibody (N-Term) (ABIN2776418)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED21 Antibody

Binding Specificity: N-Term

Purpose: Med21 Antibody - N-terminal region

Sequence: FCNAIGVLQQ CGPPASFSNI QTAINKDQPA NPTEEYAQLF AALIARTAKD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against Med21. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35728-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: Med21

Background:

Targeted Subunit: 21

Background Information: Med21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene Name: Mediator complex subunit 21

Alternative Symbols: Sr, Sur, Srb7, Surb7, D19234, AI449604, D6Ertd782, D6Ertd782e, 0610007L03Rik

Protein Name: Mediator of RNA polymerase II transcription subunit 21

Molecular Weight: 15kDa

Gene ID: 108098

NCBI Accession: NP_079591

UniProt: Q9CQ39

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha