CD48 antibody (AA 59-178)

Catalog No. ABIN7127217

This Mouse Monoclonal antibody specifically detects CD48 in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for CD48 antibody (AA 59-178) (ABIN7127217)

Target: CD48

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD48 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD48 Antibody

Binding Specificity: AA 59-178

Specificity: Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 59-178) of human CD48 protein (exact sequence is proprietary)

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Human lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD48

Target: CD48

Alternative Name: CD48

Background: B-cell activation marker, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, BLAST1, CD48 antigen (B cell membrane protein), Signaling lymphocytic activation molecule 2 (SLAMF2), SLAM family member 2,CD48 (Pan Leukocyte Marker)
Cellular localisation: Cell Surface

Molecular Weight: 45kDa

Gene ID: 962, 243564

UniProt: P09326