CD63 antibody

Catalog No. ABIN7127224

The Mouse Monoclonal anti-CD63 antibody has been validated for FACS, IF, ELISA, IP, IHC (f) and Func. It is suitable to detect CD63 in samples from Human.

Quick Overview for CD63 antibody (ABIN7127224)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Functional Studies (Func)

Product Details anti-CD63 Antibody

Specificity: CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Cross-Reactivity (Details): Human. Porcine.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full-length human CD63 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA; Functional studies; Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HL60, THP-1 or NIH/3T3 cells. Human spleen, melanoma or lymphoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: Gp55, granulophysin, Lysosomal-associated membrane protein 3, Mast cell antigen AD1Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, NGA, Ocular melanoma-associated antigen, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell surface. Cytoplasm.

Molecular Weight: 26kDa (core protein), 30-60kDa (glycosylated)

Gene ID: 967, 445570

UniProt: P08962