KRT5 & KRT6 antibody

Catalog No. ABIN7127253

This Mouse Monoclonal antibody specifically detects KRT5 & KRT6 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for KRT5 & KRT6 antibody (ABIN7127253)

Target: KRT5 & KRT6

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details

Specificity: It recognizes polypeptides of 58 kDa and 56 kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT5 protein

Isotype: IgG1, IgG2a

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details

Target: KRT5 & KRT6

Background: CK5, Keratin-5, KRT5, Cytoskeletal 6A, KRT6A,Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 58kDa (KRT5), 56kDa (KRT6A)