Recombinant Keratin 77 antibody (AA 100-200)

Catalog No. ABIN7127261

This anti-Keratin 77 antibody is a Rabbit Monoclonal antibody detecting Keratin 77 in IHC (f). Suitable for Human.

Quick Overview for Recombinant Keratin 77 antibody (AA 100-200) (ABIN7127261)

Target: Keratin 77 (KRT77)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Keratin 77 Antibody

Binding Specificity: AA 100-200

Specificity: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77

Background: K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Type I (Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 60/53/51/45/40kDa

Gene ID: 374454, 334989

UniProt: Q7Z794