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beta Actin antibody (AA 1-50)

Name: beta Actin antibody (AA 1-50)
Brand: antibodies-online
SKU: ABIN7127368
Price: 264 USD
Availability: InStock

This anti-beta Actin antibody is a Mouse Monoclonal antibody detecting beta Actin in WB, ELISA, IHC, IF, IP and FACS. Suitable for Human.

Western Blot Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate at 40 μg, 20 μg, 10 μg All lanes:ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 20 μg 293T whole cell lysate ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

9 images

Catalog No. ABIN7127368

Quick Overview for beta Actin antibody (AA 1-50) (ABIN7127368)

Target

See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This beta Actin antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone

1D12E12B5

Discover our top product ACTB Primary Antibody

Binding Specificity
41

15

15

8

6

6

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Purification

Protein A purified

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Isotype

IgG2b
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey WB, IF Host: Goat Polyclonal unconjugated

anti-Actin, beta (ACTB) (Middle Region) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse, Goat WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1 µL-5 µL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

ACTB

Background

ACTB,ACTB,ACTB,PS1TP5BP1,beta-ac6n,β-ac6n

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction