Recombinant IRAK4 antibody

Catalog No. ABIN7127580

The Rabbit Monoclonal anti-IRAK4 antibody has been validated for WB, ELISA and FACS. It is suitable to detect IRAK4 in samples from Human.

Quick Overview for Recombinant IRAK4 antibody (ABIN7127580)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 10H4

Product Details anti-IRAK4 Antibody

Cross-Reactivity: Human

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IRAK4

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, FC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background:

Background: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Aliases: Interleukin-1 receptor-associated kinase 4 (IRAK-4) (EC 2.7.11.1) (Renal carcinoma antigen NY-REN-64), IRAK4

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades