CYP7B1 antibody (AA 351-450)

Catalog No. ABIN708956

The Rabbit Polyclonal anti-CYP7B1 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect CYP7B1 in samples from Human.

Quick Overview for CYP7B1 antibody (AA 351-450) (ABIN708956)

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP7B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CYP7B1 Antibody

Binding Specificity: AA 351-450

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CYP7B1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CYP7B1

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: CYP7B1

Background:

Synonyms: 25 hydroxycholesterol 7 alpha hydroxylase, CP7B, Cytochrome P450 7B1, Cytochrome P450 family 7 subfamily B polypeptide 1, Cytochrome P450 subfamily VIIB polypeptide 1, Oxysterol 7alpha hydroxylase, CP7B1_HUMAN.

Background: The CYP7B1 protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme is likely to play a minor role in total bile acid synthesis, and may also be involved in the development of neurosteroid metabolism, atherosclerosis and sex hormone synthesis, and is a member of the cytochrome P450 superfamily of enzymes.

Gene ID: 9420

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling