EIF4G1 antibody (pSer1232)

Catalog No. ABIN7138417

The Rabbit Polyclonal anti-EIF4G1 antibody (ABIN7138417) specifically detects EIF4G1 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for EIF4G1 antibody (pSer1232) (ABIN7138417)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF4G1 Antibody

Binding Specificity: pSer1232

Purpose: Phospho-EIF4G1 (Ser1232) Antibody

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 1232 (P-V-S(p)-P-L) derived from Human eIF4G.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: EIF4G1

Background:

Background: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3, in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.

Aliases: DKFZp686A1451 antibody, eIF 4 gamma 1 antibody, eIF 4G 1 antibody, eIF 4G1 antibody, eIF-4-gamma 1 antibody, eIF-4G 1 antibody, eIF-4G1 antibody, EIF4 gamma antibody, EIF4F antibody, EIF4G antibody, EIF4G1 antibody, EIF4GI antibody, Eukaryotic translation initiation factor 4 gamma 1 antibody, IF4G1_HUMAN antibody, p220 antibody

UniProt: Q04637