ATF4 antibody (pSer219)

Catalog No. ABIN7138485

The Rabbit Polyclonal anti-ATF4 antibody is suitable to detect ATF4 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for ATF4 antibody (pSer219) (ABIN7138485)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATF4 Antibody

Binding Specificity: pSer219

Purpose: Phospho-ATF4 (Ser219) Antibody

Cross-Reactivity: Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 219 (N-D-S(p)-G-T) derived from Human ATF-4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background:

Background:

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Sato T., Shimotohno K. J. Virol. 65:1420-1426(1991)
Leiden J.M. Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992)
Bridgeman A.M. Wright H. Nature 402:489-495(1999)

Aliases: Activating transcription factor 4 antibody, ATF 4 antibody, ATF4 antibody, ATF4 protein antibody, ATF4_HUMAN antibody, cAMP-dependent transcription factor ATF-4 antibody, cAMP-responsive element-binding protein 2 antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, Cyclic AMP dependent transcription factor ATF 4 antibody, Cyclic AMP response element binding protein 2 antibody, Cyclic AMP-dependent transcription factor ATF-4 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, DNA binding protein TAXREB67 antibody, DNA-binding protein TAXREB67 antibody, Tax Responsive Enhancer Element B67 antibody, Tax-responsive enhancer element-binding protein 67 antibody, TaxREB67 antibody, TXREB antibody

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response