FBXL3 antibody (pSer320)

Catalog No. ABIN7138554

The Rabbit Polyclonal anti-FBXL3 antibody has been validated for WB and ELISA. It is suitable to detect FBXL3 in samples from Human.

Quick Overview for FBXL3 antibody (pSer320) (ABIN7138554)

Target: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FBXL3 Antibody

Binding Specificity: pSer320

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 320 (S-V-S(p)-K-D) derived from Human FBXL3.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for FBXL3

Target: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Alternative Name: FBXL3

Background:

Background:

 Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2. Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex.

 

1)Busino L., Bassermann F., Maiolica A., Lee C., Nolan P.M., Godinho S.I., Draetta G.F., Pagano M. Science 316:900-904(2007)                                          

2)Yoo S.H., Mohawk J.A., Siepka S.M., Shan Y., Huh S.K., Hong H.K., Kornblum I., Kumar V., Koike N., Xu M., Nussbaum J., Liu X., Chen Z., Chen Z.J., Green C.B., Takahashi J.S.Cell 152:1091-1105(2013)

 

Aliases: F box and leucine rich repeat protein 3 antibody; F box and leucine rich repeat protein 3A antibody; F box protein Fbl3a antibody; F-box and leucine-rich repeat protein 3A antibody; F-box/LRR-repeat protein 3 antibody; F-box/LRR-repeat protein 3A antibody; FBL3 antibody; FBL3A antibody; Fbxl3 antibody; FBXL3_HUMAN antibody; FBXL3A antibody

UniProt: Q9UKT7

Pathways: Photoperiodism