Cytokeratin 18 antibody (pSer33)

Catalog No. ABIN7138559

This Rabbit Polyclonal antibody specifically detects Cytokeratin 18 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 18 antibody (pSer33) (ABIN7138559)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: pSer33

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18

Background:

Background: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.

Aliases: Cell proliferation inducing gene 46 protein antibody, Cell proliferation inducing protein 46 antibody, Cell proliferation-inducing gene 46 protein antibody, CK 18 antibody, CK-18 antibody, CK18 antibody, CYK 18 antibody, CYK18 antibody, Cytokeratin 18 antibody, Cytokeratin endo B antibody, Cytokeratin-18 antibody, K 18 antibody, K18 antibody, K1C18_HUMAN antibody, KA18 antibody, Keratin 18 antibody, Keratin 18, type I antibody, Keratin D antibody, keratin, type I cytoskeletal 18 antibody, Keratin-18 antibody, Krt18 antibody

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis