EIF2S1 antibody (pSer49)

Catalog No. ABIN7138634

This Rabbit Polyclonal antibody specifically detects EIF2S1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human.

Quick Overview for EIF2S1 antibody (pSer49) (ABIN7138634)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-EIF2S1 Antibody

Binding Specificity: pSer49

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1

Background:

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.

Aliases: EIF 2 alpha antibody, EIF 2 antibody, EIF 2A antibody, EIF 2alpha antibody, eIF-2-alpha antibody, eIF-2A antibody, EIF-2alpha antibody, EIF2 alpha antibody, EIF2 antibody, EIF2A antibody, EIF2S1 antibody, Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa antibody, Eukaryotic translation initiation factor 2 subunit 1 alpha antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, Eukaryotic translation initiation factor 2 subunit alpha antibody, IF2A_HUMAN antibody

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C