EIF2S1 antibody (pSer51)

Catalog No. ABIN7138638

The Rabbit Polyclonal anti-EIF2S1 antibody is suitable to detect EIF2S1 in samples from Human. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for EIF2S1 antibody (pSer51) (ABIN7138638)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF2S1 Antibody

Binding Specificity: pSer51

Purpose: Phospho-EIF2S1 (Ser51) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1

Background:

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem, 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem , 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol , 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol , 26: 3955 - 3965.

Aliases: EIF 2 alpha antibody, EIF 2 antibody, EIF 2A antibody, EIF 2alpha antibody, eIF-2-alpha antibody, eIF-2A antibody, EIF-2alpha antibody, EIF2 alpha antibody, EIF2 antibody, EIF2A antibody, EIF2S1 antibody, Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa antibody, Eukaryotic translation initiation factor 2 subunit 1 alpha antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, Eukaryotic translation initiation factor 2 subunit alpha antibody, IF2A_HUMAN antibody

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C