alpha Adducin antibody (pSer726)

Catalog No. ABIN7138686

The Rabbit Polyclonal anti-alpha Adducin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect alpha Adducin in samples from Human.

Quick Overview for alpha Adducin antibody (pSer726) (ABIN7138686)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Adducin Antibody

Binding Specificity: pSer726

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: ADD1

Background:

Background: Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30, 102(35): 12407-12
Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01, 332(2): 347-51
Barkalow KL, et al. J Cell Biol 2003 May 12, 161(3): 557-70

Aliases: ADD A antibody, ADD1 antibody, ADDA antibody, ADDA_HUMAN antibody, Adducin 1 (alpha) antibody, Alpha adducin antibody, Alpha-adducin antibody, Erythrocyte adducin alpha subunit antibody, Erythrocyte adducin subunit alpha antibody, MGC3339 antibody, MGC44427 antibody, OTTHUMP00000151163 antibody, OTTHUMP00000151164 antibody, OTTHUMP00000151165 antibody, OTTHUMP00000218664 antibody, OTTHUMP00000218665 antibody

UniProt: P35611

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling