YAP1 antibody (pSer127)

Catalog No. ABIN7138743

The Rabbit Polyclonal anti-YAP1 antibody has been validated for WB and ELISA. It is suitable to detect YAP1 in samples from Human.

Quick Overview for YAP1 antibody (pSer127) (ABIN7138743)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-YAP1 Antibody

Binding Specificity: pSer127

Purpose: Phospho-YAP1 (Ser127) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP .

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background:

Background:

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.

Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66
Yang S, et al. (2014) Cell Signal 26, 343-51
Sun W, et al. (2014) Mol Biol Rep 41, 947-56

Aliases: 65 kDa Yes associated protein antibody, 65 kDa Yes-associated protein antibody, COB1 antibody, YAp 1 antibody, YAP 65 antibody, YAP antibody, YAP-1 antibody, YAP1 antibody, YAP1_HUMAN antibody, YAP2 antibody, YAP65 antibody, yes -associated protein delta antibody, Yes associated protein 1 65 kDa antibody, Yes associated protein 1 antibody, Yes associated protein 2 antibody, yes associated protein beta antibody, YKI antibody, Yorkie homolog antibody

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha